Anti-MBTD1 antibody - C-terminal (ab170848)

Overview

  • Product name
    Anti-MBTD1 antibody - C-terminal
    See all MBTD1 primary antibodies
  • Description
    Rabbit polyclonal to MBTD1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MBTD1 aa 571-600 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q05BQ5

  • Positive control
    • Human liver tissue, HL60 cell line lysate, mouse testis tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 71 kDa.

Target

  • Function
    Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility (By similarity). Specifically binds to monomethylated and dimethylated 'Lys-20' on histone H4.
  • Sequence similarities
    Contains 1 FCS-type zinc finger.
    Contains 4 MBT repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA408199 antibody
    • AI194990 antibody
    • FLJ20055 antibody
    • FLJ42067 antibody
    • Hemp antibody
    • mbt domain containing 1 antibody
    • MBT domain-containing protein 1 antibody
    • mbtd1 antibody
    • MBTD1_HUMAN antibody
    • MGC126785 antibody
    • RP23-440P13.2 antibody
    • SA49P01 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling MBTD1 using ab170848 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-MBTD1 antibody - C-terminal (ab170848) at 1/100 dilution + HL60 cell line lysate at 35 µg

    Predicted band size: 71 kDa

  • Anti-MBTD1 antibody - C-terminal (ab170848) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

    Predicted band size: 71 kDa

References

ab170848 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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