Recombinant
RabMAb

Anti-MC1 Receptor antibody [EPR6530] (ab125031)

Overview

  • Product name
    Anti-MC1 Receptor antibody [EPR6530]
    See all MC1 Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR6530] to MC1 Receptor
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MC1 Receptor.

  • Positive control
    • WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab125031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
    • Tissue specificity
      Melanocytes and corticoadrenal tissue.
    • Involvement in disease
      Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMM5 antibody
      • MC1-R antibody
      • MC1R antibody
      • Melanocortin 1 receptor antibody
      • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
      • Melanocortin receptor 1 antibody
      • Melanocyte-stimulating hormone receptor antibody
      • Melanotropin receptor antibody
      • MSH-R antibody
      • MSHR antibody
      • MSHR_HUMAN antibody
      • SHEP2 antibody
      see all

    Anti-MC1 Receptor antibody [EPR6530] images

    • All lanes : Anti-MC1 Receptor antibody [EPR6530] (ab125031) at 1/1000 dilution

      Lane 1 : A375 cell lysate
      Lane 2 : Human melanoma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 35 kDa
    • ab125031, at a 1/500 dilution, staining MC1 Receptor in paraffin embedded Human melanoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-MC1 Receptor antibody [EPR6530] (ab125031)

    ab125031 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsy and cells)
    Loading amount
    20 µg
    Specification
    skin biopsy and cells
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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