The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Mouse MC4 Receptor peptide (ab78212). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein.
Use a concentration of 1 µg/ml.
Use a concentration of 0.4 µg/ml.
FunctionReceptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificityBrain, placental, and gut tissues.
Involvement in diseaseDefects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Immunohistochemistry (Frozen sections) - Anti-MC4 Receptor antibody (ab24233)This image is courtesy of an Abreview submitted by Thomas Groves
ab24233 staining MC4 Receptor in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in TPBS + normal goat serum) for 19 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Immunoperoxidase staining of rat brain section with ab24233. Note the tadpole like staining of cortex neurons.
References for Anti-MC4 Receptor antibody (ab24233)
This product has been referenced in:
Peter JC et al. A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. J Pharmacol Exp Ther333:478-90 (2010).
Read more (PubMed: 20118207) »
Chitravanshi VC et al. Microinjections of alpha-melanocyte stimulating hormone into the nucleus ambiguus of the rat elicit vagally mediated bradycardia. Am J Physiol Regul Integr Comp Physiol296:R1402-11 (2009).
Read more (PubMed: 19297540) »