• Product nameAnti-MC4 Receptor antibody
    See all MC4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to MC4 Receptor
  • SpecificityThis antibody detects endogenous levels of total MC4 Receptor protein.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from an internal region of human MC4 Receptor

  • Positive control
    • Extracts from MCF-7 cells This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U-87 MG.



Our Abpromise guarantee covers the use of ab75506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA 1/40000.


  • FunctionReceptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
  • Tissue specificityBrain, placental, and gut tissues.
  • Involvement in diseaseDefects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MC4-R antibody
    • MC4R antibody
    • MC4R_HUMAN antibody
    • Melanocortin 4 receptor antibody
    • Melanocortin receptor 4 antibody
    • MGC126851 antibody
    • MGC138197 antibody
    see all

Anti-MC4 Receptor antibody images

  • ab75506 stained U-87 MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75506 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MC4 Receptor antibody (ab75506) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from MCF-7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • IHC-P image of MC4 Receptor staining on human placenta sections using ab75506 (1:1000). The sections were de-paraffinized and subjected to heat mediated antigen retrieval using Citric acid pH6. The sections were blocked using 1% BSA for 10 mins at 21°C. Primary antibody was incubated at 21°C for 2 hours.

    See Abreview

References for Anti-MC4 Receptor antibody (ab75506)

This product has been referenced in:
  • Simamura E  et al. Melanocortins contribute to sequential differentiation and enucleation of human erythroblasts via melanocortin receptors 1, 2 and 5. PLoS One 10:e0123232 (2015). Read more (PubMed: 25860801) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brains 25um sections)
Specification brains 25um sections
Fixative formalin
Permeabilization Yes - 1xPBT
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C

Abcam user community

Verified customer

Submitted Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Sep 03 2012