FunctionReceptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificityBrain, placental, and gut tissues.
Involvement in diseaseDefects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab75506 stained U-87 MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75506 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - MC4 Receptor antibody (ab75506)
All lanes : Anti-MC4 Receptor antibody (ab75506) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells Lane 2 : Extracts from MCF-7 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 37 kDa Observed band size : 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC4 Receptor antibody (ab75506)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
IHC-P image of MC4 Receptor staining on human placenta sections using ab75506 (1:1000). The sections were de-paraffinized and subjected to heat mediated antigen retrieval using Citric acid pH6. The sections were blocked using 1% BSA for 10 mins at 21°C. Primary antibody was incubated at 21°C for 2 hours.
References for Anti-MC4 Receptor antibody (ab75506)
This product has been referenced in:
Simamura E et al. Melanocortins contribute to sequential differentiation and enucleation of human erythroblasts via melanocortin receptors 1, 2 and 5. PLoS One10:e0123232 (2015).
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