Overview

  • Product nameAnti-MC4 Receptor antibody
    See all MC4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to MC4 Receptor
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MC4 Receptor.

  • Positive control
    • This antibody gave a positive signal in both Caco2 and JEG3 whole cell lysates as well as the following Human tissue lysates: Brain; Spinal Cord; Placenta; Small Intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab97432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

Anti-MC4 Receptor antibody images

  • All lanes : Anti-MC4 Receptor antibody (ab97432) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 100 kDa,70 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-MC4 Receptor antibody (ab97432)

ab97432 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"