Overview

  • Product name
    Anti-MC4 Receptor antibody
    See all MC4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to MC4 Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MC4 Receptor.

  • Positive control
    • This antibody gave a positive signal in both Caco2 and JEG3 whole cell lysates as well as the following Human tissue lysates: Brain; Spinal Cord; Placenta; Small Intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab97432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

Images

  • All lanes : Anti-MC4 Receptor antibody (ab97432) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa
    Additional bands at: 100 kDa, 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab97432 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab97432 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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