This antibody gave a positive signal in both Caco2 and JEG3 whole cell lysates as well as the following Human tissue lysates: Brain; Spinal Cord; Placenta; Small Intestine.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
FunctionReceptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificityBrain, placental, and gut tissues.
Involvement in diseaseDefects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Western blot - Anti-MC4 Receptor antibody (ab97432)
All lanes : Anti-MC4 Receptor antibody (ab97432) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) Lane 3 : Human placenta tissue lysate - total protein (ab29745) Lane 4 : Human small intestine tissue lysate - total protein (ab29276) Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 37 kDa Observed band size : 37 kDa Additional bands at : 100 kDa,70 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for Anti-MC4 Receptor antibody (ab97432)
has not yet been referenced specifically in any publications.
Publishing research using ab97432? Please let us know so that we can cite the reference in this datasheet.
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