Overview

  • Product name
    Anti-MC5 Receptor antibody
    See all MC5 Receptor primary antibodies
  • Description
    Rabbit polyclonal to MC5 Receptor
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Hamster, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MC5 Receptor.

  • Positive control
    • This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).

Target

Images

  • Lane 1 : Anti-MC5 Receptor antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
    Lane 2 : Anti-MC5 Receptor antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

References

ab87470 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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