The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
Expressed in the brain but not in the melanoma cells.
Belongs to the G-protein coupled receptor 1 family.