- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MCAF1 antibody [2052C2a]
See all MCAF1 primary antibodies -
DescriptionMouse monoclonal [2052C2a] to MCAF1
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment (Human), amino acids 50-200.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (3.0 mM KCl, 1.5 mM KH2PO4 ,
140 mM NaCl, 8.0 mM Na2HPO4), pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
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ClonalityMonoclonal
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Clone number2052C2a
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab50956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 136 kDa). |
Target
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RelevanceMCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 55729 Human
- SwissProt: Q6VMQ6 Human
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Alternative names
- Activating transcription factor 7 interacting protein 1 antibody
- ATF interacting protein antibody
- ATF IP antibody
see all
Images
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![Western blot - Anti-MCAF1 antibody [2052C2a] (ab50956)](http://a.abcam.com/ps/products/50/ab50956/Images/ab50956_1.jpg)
Western blot - Anti-MCAF1 antibody [2052C2a] (ab50956)Left: Western blot analysis of immunized recombinant protein using ab50956.Right: CBB staining of immunized recombinant protein.
Protocols
References
ab50956 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"