Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (3.0 mM KCl, 1.5 mM KH2PO4 ,
140 mM NaCl, 8.0 mM Na2HPO4), pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 136 kDa).
RelevanceMCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.