Anti-MCAF1 antibody [2052C2a] (ab50956)

Overview

• Product name
  Anti-MCAF1 antibody [2052C2a]
  See all MCAF1 primary antibodies
• Description
  Mouse monoclonal [2052C2a] to MCAF1
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment (Human), amino acids 50-200.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS (3.0 mM KCl, 1.5 mM KH2PO4 ,
  140 mM NaCl, 8.0 mM Na2HPO4), pH 7.4
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Clonality
  Monoclonal
• Clone number
  2052C2a
• Isotype
  IgG1
• Research areas
  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • Methylated DNA Binding

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Relevance
  MCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.
• Cellular localization
  Nuclear
• Database links
  • Entrez Gene: 55729 Human
  • SwissProt: Q6VMQ6 Human
• Alternative names
  • Activating transcription factor 7 interacting protein 1 antibody
  • ATF interacting protein antibody
  • ATF IP antibody

  • ATF7IP antibody
  • ATFa associated modulator antibody
  • FLJ10139 antibody
  • FLJ10688 antibody
  • hAM antibody
  • HGNC:20092 antibody
  • MBD1 containing chromatin associated factor antibody
  • MCAF 1 antibody
  • MCAF antibody
  • p621 antibody

  see all

Protocols

• Western blot protocols