Overview

  • Product nameAnti-MCAF1 antibody
    See all MCAF1 primary antibodies
  • Description
    Rabbit polyclonal to MCAF1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 25 and 100 of Human MCAF1 (AAQ92978.1).

  • Positive control
    • Human small cell lung cancer. Mouse squamous cell carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Activating transcription factor 7 interacting protein 1 antibody
      • ATF interacting protein antibody
      • ATF IP antibody
      • ATF7IP antibody
      • ATFa associated modulator antibody
      • FLJ10139 antibody
      • FLJ10688 antibody
      • hAM antibody
      • HGNC:20092 antibody
      • MBD1 containing chromatin associated factor antibody
      • MCAF 1 antibody
      • MCAF antibody
      • p621 antibody
      see all

    Anti-MCAF1 antibody images

    • ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded human small cell lung cancer by Immunohistochemistry, using DAB staining.
    • ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded mouse squamous cell carcinoma by Immunohistochemistry, using DAB staining.

    References for Anti-MCAF1 antibody (ab84497)

    This product has been referenced in:
    • Minkovsky A  et al. The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation. Epigenetics Chromatin 7:12 (2014). Mouse . Read more (PubMed: 25028596) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"