• Product nameAnti-MCAK antibody [2488C3a]
    See all MCAK primary antibodies
  • Description
    Mouse monoclonal [2488C3a] to MCAK
  • Tested applicationsSuitable for: WB, Dot Blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human MCAK fragment


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4).
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • ClonalityMonoclonal
  • Clone number2488C3a
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab50778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 81 kDa.
Dot Blot Use at an assay dependent dilution.


  • FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Developmental stageIsoform 2 is expressed in fetal testis.
  • DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930402F02Rik antibody
    • CT139 antibody
    • ESTM5 antibody
    • KIF 2C antibody
    • kif2c antibody
    • KIF2C_HUMAN antibody
    • Kinesein Family Member 2C antibody
    • Kinesin family member 2C antibody
    • kinesin like 6 (mitotic centromere associated kinesin) antibody
    • Kinesin like 6 antibody
    • Kinesin like protein 6 antibody
    • Kinesin like protein KIF2C antibody
    • Kinesin-like protein 6 antibody
    • Kinesin-like protein KIF2C antibody
    • KNS L6 antibody
    • KNSL 6 antibody
    • KNSL6 antibody
    • MCAK antibody
    • MGC11883 antibody
    • Mitotic centromere associated kinesin antibody
    • Mitotic centromere-associated kinesin antibody
    • OTTHUMP00000010066 antibody
    • X83316 antibody
    see all

Anti-MCAK antibody [2488C3a] images

  • Anti-MCAK antibody [2488C3a] (ab50778) + immunizing peptide

    Predicted band size : 81 kDa

References for Anti-MCAK antibody [2488C3a] (ab50778)

This product has been referenced in:
  • Braun A  et al. Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells. J Cell Biol 206:97-112 (2014). Read more (PubMed: 25002679) »

See 1 Publication for this product

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