Overview

  • Product name
  • Description
    Rabbit polyclonal to MCAK
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chimpanzee, Ferret, Rhesus monkey, Chinese hamster
  • Immunogen

    Synthetic peptide from a region between residue 50 and 100 of Human MCAK using the numbering given in entry NP_006836.1

  • Positive control
    • Whole lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
    • Tissue specificity
      Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
    • Sequence similarities
      Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
      Contains 1 kinesin-motor domain.
    • Developmental stage
      Isoform 2 is expressed in fetal testis.
    • Domain
      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
    • Cellular localization
      Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930402F02Rik antibody
      • CT139 antibody
      • ESTM5 antibody
      • KIF 2C antibody
      • kif2c antibody
      • KIF2C_HUMAN antibody
      • Kinesein Family Member 2C antibody
      • Kinesin family member 2C antibody
      • kinesin like 6 (mitotic centromere associated kinesin) antibody
      • Kinesin like 6 antibody
      • Kinesin like protein 6 antibody
      • Kinesin like protein KIF2C antibody
      • Kinesin-like protein 6 antibody
      • Kinesin-like protein KIF2C antibody
      • KNS L6 antibody
      • KNSL 6 antibody
      • KNSL6 antibody
      • MCAK antibody
      • MGC11883 antibody
      • Mitotic centromere associated kinesin antibody
      • Mitotic centromere-associated kinesin antibody
      • OTTHUMP00000010066 antibody
      • X83316 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and breast carcinoma (right) tissues labelling MCAK with ab70536 at 1/1000 (1µg/ml). Detection: DAB.

    References

    ab70536 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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