Recombinant Anti-MCAK antibody [EPR14838] (ab187652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14838] to MCAK
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MCAK antibody [EPR14838]
See all MCAK primary antibodies -
Description
Rabbit monoclonal [EPR14838] to MCAK -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF7, 293T, Hela and K562 cell lysates. Hela and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14838 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/260.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 81 kDa.
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Notes |
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Flow Cyt (Intra)
1/260. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
WB
1/1000 - 1/2000. Predicted molecular weight: 81 kDa. |
Target
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Function
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
Tissue specificity
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
Sequence similarities
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
Developmental stage
Isoform 2 is expressed in fetal testis. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 11004 Human
- Omim: 604538 Human
- SwissProt: Q99661 Human
- Unigene: 720061 Human
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Alternative names
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
Images
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Intracellular flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
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All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : Hela cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa -
Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
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Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab187652 has been referenced in 4 publications.
- Li Q et al. CKAP2L, a crucial target of miR-326, promotes prostate cancer progression. BMC Cancer 22:666 (2022). PubMed: 35715760
- Marquis C et al. Chromosomally unstable tumor cells specifically require KIF18A for proliferation. Nat Commun 12:1213 (2021). PubMed: 33619254
- Hadders MA et al. Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis. J Cell Biol 219:N/A (2020). PubMed: 32027339
- Sellerio AL et al. Overshoot during phenotypic switching of cancer cell populations. Sci Rep 5:15464 (2015). PubMed: 26494317