Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. Contains 1 kinesin-motor domain.
Isoform 2 is expressed in fetal testis.
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
kinesin like 6 (mitotic centromere associated kinesin) antibody
Kinesin like 6 antibody
Kinesin like protein 6 antibody
Kinesin like protein KIF2C antibody
Kinesin-like protein 6 antibody
Kinesin-like protein KIF2C antibody
KNS L6 antibody
KNSL 6 antibody
Mitotic centromere associated kinesin antibody
Mitotic centromere-associated kinesin antibody
Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HEK293 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.