• Product name
    Anti-MCAK antibody [EPR14838]
    See all MCAK primary antibodies
  • Description
    Rabbit monoclonal [EPR14838] to MCAK
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MCAK aa 50-150. The exact sequence is proprietary.
    Database link: Q99661

  • Positive control
    • MCF7, 293T, Hela and K562 cell lysates. Hela and MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab187652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/260.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF 1/100.
WB 1/1000 - 1/2000. Predicted molecular weight: 81 kDa.


  • Function
    Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • Tissue specificity
    Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • Sequence similarities
    Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Developmental stage
    Isoform 2 is expressed in fetal testis.
  • Domain
    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930402F02Rik antibody
    • CT139 antibody
    • ESTM5 antibody
    • KIF 2C antibody
    • kif2c antibody
    • KIF2C_HUMAN antibody
    • Kinesein Family Member 2C antibody
    • Kinesin family member 2C antibody
    • kinesin like 6 (mitotic centromere associated kinesin) antibody
    • Kinesin like 6 antibody
    • Kinesin like protein 6 antibody
    • Kinesin like protein KIF2C antibody
    • Kinesin-like protein 6 antibody
    • Kinesin-like protein KIF2C antibody
    • KNS L6 antibody
    • KNSL 6 antibody
    • KNSL6 antibody
    • MCAK antibody
    • MGC11883 antibody
    • Mitotic centromere associated kinesin antibody
    • Mitotic centromere-associated kinesin antibody
    • OTTHUMP00000010066 antibody
    • X83316 antibody
    see all


  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE.  Ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.


  • Flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

  • All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 81 kDa

  • Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

  • Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.


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