The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
1/500 - 1/1000.
FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily. Contains 1 kinesin-motor domain.
Developmental stageIsoform 2 is expressed in fetal testis.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
ab74146, at 1/500 dilution, staining A549 cells using immunofluorescence analysis, in the absence and presence of the immunising phosphopeptide.
Western blot - MCAK (phospho S95) antibody (ab74146)
All lanes : Anti-MCAK (phospho S95) antibody (ab74146) at 1/500 dilution
Lane 1 : HeLa cell extract, treated
with TNF (10ng/ml, 30mins), Lane 2 : HeLa cell extract, treated
with TNF (10ng/ml, 30mins), with immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 81 kDa Observed band size : 81 kDa
References for Anti-MCAK (phospho S95) antibody (ab74146)
This product has been referenced in:
Lin BW et al. Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B. Cell Death Dis5:e1106 (2014).
Read more (PubMed: 24603334) »
Pakala SB et al. Signaling-dependent phosphorylation of mitotic centromere-associated kinesin regulates microtubule depolymerization and its centrosomal localization. J Biol Chem287:40560-9 (2012).
Read more (PubMed: 23055517) »