• Product nameAnti-MCAK (phospho S95) antibody
    See all MCAK primary antibodies
  • Description
    Rabbit polyclonal to MCAK (phospho S95)
  • SpecificityAntibody detects endogenous levels of MCAK when only phosphorylated at serine 95.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human MCAK around the phosphorylation site of serine 95 (R-R-SP-V-N).

  • Positive control
    • Extract from HeLa cells (treated with TNF at 10ng/ml for 30mins). A549 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.


  • FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Developmental stageIsoform 2 is expressed in fetal testis.
  • DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930402F02Rik antibody
    • CT139 antibody
    • ESTM5 antibody
    • KIF 2C antibody
    • kif2c antibody
    • KIF2C_HUMAN antibody
    • Kinesein Family Member 2C antibody
    • Kinesin family member 2C antibody
    • kinesin like 6 (mitotic centromere associated kinesin) antibody
    • Kinesin like 6 antibody
    • Kinesin like protein 6 antibody
    • Kinesin like protein KIF2C antibody
    • Kinesin-like protein 6 antibody
    • Kinesin-like protein KIF2C antibody
    • KNS L6 antibody
    • KNSL 6 antibody
    • KNSL6 antibody
    • MCAK antibody
    • MGC11883 antibody
    • Mitotic centromere associated kinesin antibody
    • Mitotic centromere-associated kinesin antibody
    • OTTHUMP00000010066 antibody
    • X83316 antibody
    see all

Anti-MCAK (phospho S95) antibody images

  • ab74146, at 1/500 dilution, staining A549 cells using immunofluorescence analysis, in the absence and presence of the immunising phosphopeptide.
  • All lanes : Anti-MCAK (phospho S95) antibody (ab74146) at 1/500 dilution

    Lane 1 : HeLa cell extract, treated with TNF (10ng/ml, 30mins),
    Lane 2 : HeLa cell extract, treated with TNF (10ng/ml, 30mins), with immunising phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 81 kDa
    Observed band size : 81 kDa

References for Anti-MCAK (phospho S95) antibody (ab74146)

This product has been referenced in:
  • Lin BW  et al. Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B. Cell Death Dis 5:e1106 (2014). WB ; Human . Read more (PubMed: 24603334) »
  • Pakala SB  et al. Signaling-dependent phosphorylation of mitotic centromere-associated kinesin regulates microtubule depolymerization and its centrosomal localization. J Biol Chem 287:40560-9 (2012). ICC/IF ; Human . Read more (PubMed: 23055517) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 50 µg
Treatment Nocodazole 16 hrs
Specification MEF
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Feb 18 2016