General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 1 nM Estradiol for 96 hours at 37°C immediately prior to harvesting.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
BackgroundMCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
References for MCF7 nuclear extract lysate (estradiol treated) (ab14861)
has not yet been referenced specifically in any publications.
Publishing research using ab14861? Please let us know so that we can cite the reference in this datasheet.
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