For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length His tagged mCherry purified from E. coli.
Our Abpromise guarantee covers the use of ab125096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 28 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 (with a red secondary). DAPI was used to stain DNA meaning only their nuclei are visualised in blue.
ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
ab125096 staining mCherry in human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with BSA + milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.