Overview

  • Product nameAnti-MCL1 antibody [2E11]
    See all MCL1 primary antibodies
  • Description
    Mouse monoclonal [2E11] to MCL1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human MCL1 produced in HEK293T cells (NP_068779)

  • Positive control
    • WB: Transfected HEK293T cell lysate IHC-P: Breast tissue (normal and adenocarcinoma), colon tissue (normal and adenocarcinoma), kidney tissue, lung tissue, adenocarcinoma of ovary tissue, pancreas tissue (normal and carcinoma), bladder tissue, prostate tissue and thyroid tissue (normal and carcinoma) ICC/IF: Transfected COS7 cells, HepG2 cells Flow Cyt: Transfected HEK293T cells, Hela cells, Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab114026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 37 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Post-translational
    modifications
    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Anti-MCL1 antibody [2E11] images

  • All lanes : Anti-MCL1 antibody [2E11] (ab114026) at 1/2000 dilution

    Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed
    Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY MCL1 cDNA for 48 hrs and lysed

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 37 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200521)
  • Immunohistochemical staining of paraffin embedded breast tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Adenocarcinoma of breast tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded colon tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Adenocarcinoma of colon tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Kidney tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded lung tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Adenocarcinoma of ovary tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded pancreas tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Carcinoma of pancreas tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded thyroid tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded Carcinoma of thyroid tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded prostate tissue using ab114026 at 1/50
  • Immunohistochemical staining of paraffin embedded bladder tissue using ab114026 at 1/50
  • Immunofluorescent staining of HepG2 cells, using ab114026 at 1/100
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1, using ab114026 at 1/100
  • Flow cytometric analysis of Jurkat cells, using ab114026 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue)
  • Flow cytometric analysis of Hela cells, using ab114026 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue)
  • HEK293T cells transfected with either pCMV6-ENTRY MCL1 (Red) or empty vector control plasmid (Blue) were immunostained with ab114026 at 1/100 and then analyzed by flow cytometry

References for Anti-MCL1 antibody [2E11] (ab114026)

ab114026 has not yet been referenced specifically in any publications.

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