Overview

  • Product nameAnti-MCL1 antibody
    See all MCL1 primary antibodies
  • Description
    Rabbit polyclonal to MCL1
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human MCL1.

  • Positive control
    • Human breast carcinoma tissue Extracts from HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab53709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Post-translational
    modifications
    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Anti-MCL1 antibody images

  • All lanes : Anti-MCL1 antibody (ab53709) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells minus the immunising peptide
    Lane 2 : Extracts from HuvEc cells plus the immunising peptide


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
  • ab53709, at a 1/50 dilution, minus immunising peptide, staining Human MCL1 in Breast Carcinoma, using Immunohistochemistry, Paraffin embedded tissue.
  • ab53709, at a 1/50 dilution, plus immunising peptide, staining Human MCL1 in Breast Carcinoma, using Immunohistochemistry, Paraffin embedded tissue.
  • ICC/IF image of ab53709 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53709, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MCL1 antibody (ab53709)

This product has been referenced in:
  • Nipic D  et al. Preapoptotic cell stress response of primary hepatocytes. Hepatology 51:2140-51 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20513000) »
  • Rodenberg JM  et al. Regulation of serum response factor activity and smooth muscle cell apoptosis by chromodomain helicase DNA-binding protein 8. Am J Physiol Cell Physiol 299:C1058-67 (2010). WB ; Mouse . Read more (PubMed: 20739623) »

See all 2 Publications for this product

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