Anti-MCL1 antibody [RC13] (Biotin) (ab79473)

Overview

  • Product nameAnti-MCL1 antibody [RC13] (Biotin)
    See all MCL1 primary antibodies
  • Description
    Mouse monoclonal [RC13] to MCL1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1 - 327 of Human MCL1

  • Positive control
    • Raji cell lysate. Spleen or tonsil tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab79473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notesIHC-P: 1/50, for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, before immunostaining.
    IP: (denatured, with protein G) Use at 2 µg/mg of lysate.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 37 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
    • Sequence similaritiesBelongs to the Bcl-2 family.
    • Post-translational
      modifications
      Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
      Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
      Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
    • Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bcl 2 related protein EAT/mcl1 antibody
      • Bcl-2-like protein 3 antibody
      • Bcl-2-related protein EAT/mcl1 antibody
      • BCL2 related antibody
      • Bcl2-L-3 antibody
      • BCL2L3 antibody
      • EAT antibody
      • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
      • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
      • MCL 1 antibody
      • MCL1 antibody
      • MCL1-ES antibody
      • mcl1/EAT antibody
      • MCL1_HUMAN antibody
      • MCL1L antibody
      • MCL1S antibody
      • MGC104264 antibody
      • MGC1839 antibody
      • Myeloid Cell Leukemia 1 antibody
      • Myeloid cell leukemia ES antibody
      • Myeloid cell leukemia sequence 1 antibody
      • Myeloid cell leukemia sequence 1 BCL2 related antibody
      • Myeloid cell leukemia sequence 1 isoform 1 antibody
      • OTTHUMP00000032794 antibody
      • OTTHUMP00000032795 antibody
      • TM antibody
      see all

    References for Anti-MCL1 antibody [RC13] (Biotin) (ab79473)

    ab79473 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"