Anti-MCL1 (phospho S159) antibody (ab111574)

Overview

  • Product name
    Anti-MCL1 (phospho S159) antibody
    See all MCL1 primary antibodies
  • Description
    Rabbit polyclonal to MCL1 (phospho S159)
  • Specificity
    ab111574 detects endogenous levels of MCL1 only when phosphorylated at serine 159.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from Human MCL1 around the phosphorylation site of Serine 159 (D-G-SP-L-P).

  • Positive control
    • A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab111574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Post-translational
    modifications
    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localization
    Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Images

  • ab111574, at 1/100 dilution, staining MCL1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:
  • Sarabhai T  et al. Serum a-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PLoS One 12:e0177450 (2017). WB . Read more (PubMed: 28493974) »
  • Weigel KJ  et al. CAF-secreted IGFBPs regulate breast cancer cell anoikis. Mol Cancer Res 12:855-66 (2014). Read more (PubMed: 24803643) »

See all 2 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Liver cells)
Loading amount
80 µg
Specification
Liver cells
Treatment
DMSO 24 h or tBHQ different time periods
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jun 05 2012

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I am very pleased to hear you would like to accept our offer and test ab111574 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiratio...

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I have confirmed that the antibody ab111574, raised against MCL1 pSer159, has not been tested for reactivity with MCL1 pSer159/pThr163.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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