Anti-MCL1 (phospho S159) antibody (ab111574)

Overview

  • Product nameAnti-MCL1 (phospho S159) antibody
    See all MCL1 primary antibodies
  • Description
    Rabbit polyclonal to MCL1 (phospho S159)
  • Specificityab111574 detects endogenous levels of MCL1 only when phosphorylated at serine 159.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from Human MCL1 around the phosphorylation site of Serine 159 (D-G-SP-L-P).

  • Positive control
    • A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab111574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/500.

Target

  • FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • Post-translational
    modifications
    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Anti-MCL1 (phospho S159) antibody images

  • ab111574, at 1/100 dilution, staining MCL1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-MCL1 (phospho S159) antibody (ab111574)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting us.

I have replaced the image with the one you just sent me, and copied the figure legend into the Additional Notes box on the Abreview. The review will be published shortly.

Thank you for testing the antib...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Liver cells)
Loading amount 80 µg
Specification Liver cells
Treatment DMSO 24 h or tBHQ different time periods
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 05 2012

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab111574 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiratio...

Read More

I have confirmed that the antibody ab111574, raised against MCL1 pSer159, has not been tested for reactivity with MCL1 pSer159/pThr163.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"