Overview

  • Product nameAnti-MCM-BP antibody
    See all MCM-BP primary antibodies
  • Description
    Rabbit polyclonal to MCM-BP
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: SPRNTTLERQ TFYCVPVPGE STWVKEAYVN ANQARVSPST SYTPSRHKRS YEDDDDMDLQ PNKQKDQHAG ARQAGSVGGL QWCGEPKRLE TEASTGQ, corresponding to internal sequence amino acids 118-214 of Human MCM-BP.

  • Positive control
    • Human stomach tissue; RT4, U 251 MG, Human plasma, liver and tonsil lysates; A431s.

Properties

Applications

Our Abpromise guarantee covers the use of ab122478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • FunctionAssociated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
  • Sequence similaritiesBelongs to the MCMBP family.
  • Cellular localizationNucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • C10orf119 antibody
    • chromosome 10 open reading frame 119 antibody
    • MCM-binding protein antibody
    • MCM-BP antibody
    • mcmbp antibody
    • MCMBP_HUMAN antibody
    • Mini-chromosome maintenance complex-binding protein antibody
    • Minichromosome maintenance complex binding protein antibody
    see all

Anti-MCM-BP antibody images

  • ab122478 at 1/50 dilution staining MCM-BP in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122478 at 4 µg/ml staining MCM-BP in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
  • All lanes : Anti-MCM-BP antibody (ab122478) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References for Anti-MCM-BP antibody (ab122478)

ab122478 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"