Overview

  • Product name
  • Description
    Rabbit polyclonal to MCM10
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 40-89 (DEFDELFDAD GDGESYTEEA GSGEEGKTGN QEERLATLFG DVEDLTDDEV) of Mouse MCM10 (NP_081566).

  • Positive control
    • Mouse pancreas lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Constituents: 98% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 98 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
  • Sequence similarities
    Belongs to the MCM10 family.
  • Developmental stage
    Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
  • Domain
    The zinc finger-like domain binds a zinc ion and is involved in self-association.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNA43 antibody
    • DNA43 antibody
    • FLJ31133 antibody
    • Homolog of yeast MCM10 antibody
    • HsMCM10 antibody
    • mcm10 antibody
    • MCM10 minichromosome maintenance deficient 10 antibody
    • MCM10_HUMAN antibody
    • Minichromosome maintenance 10 replication initiation factor antibody
    • Minichromosome maintenance complex component 10 antibody
    • Minichromosome maintenance protein 10 isoform 1 antibody
    • PRO2249 antibody
    • Protein MCM10 homolog antibody
    see all

Images

  • Anti-MCM10 antibody (ab116072) at 1 µg/ml + Mouse pancreas lysate at 10 µg

    Predicted band size : 98 kDa

References

ab116072 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab116072.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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