Anti-MCM10 antibody (ab230109)
Key features and details
- Rabbit polyclonal to MCM10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MCM10 antibody
See all MCM10 primary antibodies -
Description
Rabbit polyclonal to MCM10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MCM10 aa 600 to the C-terminus.
Database link: Q7L590 -
Positive control
- IHC-P: Human tonsil and spleen tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
Notes |
---|
IHC-P
1/20 - 1/200. |
Target
-
Function
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. -
Sequence similarities
Belongs to the MCM10 family. -
Developmental stage
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase. -
Domain
The zinc finger-like domain binds a zinc ion and is involved in self-association. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli. - Information by UniProt
-
Database links
- Entrez Gene: 55388 Human
- Omim: 609357 Human
- SwissProt: Q3MIR3 Human
- SwissProt: Q5T670 Human
- SwissProt: Q7L590 Human
- SwissProt: Q9H0D7 Human
- SwissProt: Q9H3P9 Human
- Unigene: 198363 Human
-
Alternative names
- CNA43 antibody
- DNA43 antibody
- FLJ31133 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab230109 has been referenced in 1 publication.
- Chen Z et al. Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma. Dig Surg 39:24-31 (2022). PubMed: 34788753