• Product nameAnti-MCM10 antibody
    See all MCM10 primary antibodies
  • Description
    Rabbit polyclonal to MCM10
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, OrangutanDoes not react with: Xenopus laevis, Drosophila melanogaster
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.

  • Positive control
    • HeLa cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).


  • FunctionActs as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
  • Sequence similaritiesBelongs to the MCM10 family.
  • Developmental stageExpression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
  • DomainThe zinc finger-like domain binds a zinc ion and is involved in self-association.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31133 antibody
    • HsMCM10 antibody
    • mcm10 antibody
    • MCM10 minichromosome maintenance deficient 10 antibody
    • MCM10_HUMAN antibody
    • Minichromosome maintenance protein 10 isoform 1 antibody
    • PRO2249 antibody
    • Protein MCM10 homolog antibody
    see all

Anti-MCM10 antibody images

  • Predicted band size : 110 kDa

    Sample: Hela cell lysate (1mg for IP).

    Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

    Detection: ECL.

References for Anti-MCM10 antibody (ab3733)

This product has been referenced in:
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . Read more (PubMed: 24726359) »
  • Im JS  et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). Read more (PubMed: 19805216) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
Loading amount 20 µg
Specification HeLa cells and BJ human fibroblast cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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Submitted Jun 03 2008