Overview

  • Product nameAnti-MCM2 antibody [CRCT2.1]See all MCM2 primary antibodies ...
  • Description
    Mouse monoclonal [CRCT2.1] to MCM2
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterially expressed human MCM2

  • EpitopeNot determined
  • Positive control
    • HeLa cells. Tonsil

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberCRCT2.1
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab81706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: 1/50 for 20-30 minutes depending on detection system used. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    WB: Use at a concentration of 2 - 4 µg/ml for 2 hours at room temperature. Predicted molecular weight: 102 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • Sequence similaritiesBelongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • CDC like-1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • cyclin like-1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 antibody [CRCT2.1] images

    • Formalin fixed, paraffin embedded human tonsil tissue stained with anti MCM2 (form of ab81706 containing BSA and azide) at a dilution of 1/50. Peroxidase conjugate and AEC chromogen were used. Note nuclear staining of lymphocytes.
    • anti MCM2 (form of ab81706 containing BSA and azide) at 4 µg/ml + human cells

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    References for Anti-MCM2 antibody [CRCT2.1] (ab81706)

    ab81706 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab81706.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"