The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/50 for 20-30 minutes depending on detection system used. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
WB: Use at a concentration of 2 - 4 µg/ml for 2 hours at room temperature. Predicted molecular weight: 102 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similaritiesBelongs to the MCM family. Contains 1 MCM domain.
Post-translational modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Formalin fixed, paraffin embedded human tonsil tissue stained with anti MCM2 (form of ab81706 containing BSA and azide) at a dilution of 1/50. Peroxidase conjugate and AEC chromogen were used. Note nuclear staining of lymphocytes.
Western blot - MCM2 antibody [CRCT2.1] - BSA and Azide free (ab81706)
anti MCM2 (form of ab81706 containing BSA and azide) at 4 µg/ml + human cells
Predicted band size : 102 kDa Observed band size : 102 kDa
References for Anti-MCM2 antibody [CRCT2.1] (ab81706)
has not yet been referenced specifically in any publications.
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