Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

Overview

  • Product nameAnti-MCM2 antibody [CRCT2.1 (D1.9H5) ]See all MCM2 primary antibodies ...
  • Description
    Mouse monoclonal [CRCT2.1 (D1.9H5) ] to MCM2
  • SpecificityRecognises human Mcm2
  • Tested applicationsIHC-P, IHC-Fr, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterially expressed human Mcm2

  • Positive control
    • Tonsil, any tissue with high proliferative index
  • General notesClone Number: CRCT2.1 (D1.9H5)

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • Purification notesPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • Clonality Monoclonal
  • Clone numberCRCT2.1 (D1.9H5)
  • MyelomaSp2
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/500 - 1/1000.
WB Use at an assay dependent dilution. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BM28 antibody
    • CCNL 1 antibody
    • CCNL1 antibody
    • CDC like 1 antibody
    • CDC like-1 antibody
    • cdc19 antibody
    • CDCL 1 antibody
    • CDCL1 antibody
    • Cell devision cycle like 1 antibody
    • Cyclin like 1 antibody
    • cyclin like-1 antibody
    • D3S3194 antibody
    • DNA replication licensing factor MCM2 antibody
    • KIAA0030 antibody
    • MCM 2 antibody
    • MCM2 antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin antibody
    • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
    • MCM2 minichromosome maintenance deficient 2, mitotin antibody
    • MCM2_HUMAN antibody
    • MGC10606 antibody
    • Minichromosome maintenance complex component 2 antibody
    • Minichromosome maintenance deficient 2 (mitotin) antibody
    • Minichromosome maintenance deficient 2 mitotin antibody
    • Minichromosome maintenance protein 2 antibody
    • Minichromosome maintenance protein 2 homolog antibody
    • Mitotin antibody
    • Nuclear protein BM28 antibody
    • OTTHUMP00000216047 antibody
    • OTTHUMP00000216050 antibody
    see all

Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] images



  • Predicted band size : 100 kDa


    Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

References for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

This product has been referenced in:
  • Shtutman M  et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . Read more (PubMed: 20368428) »
  • Sarfraz S  et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . Read more (PubMed: 18680610) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Kidney biopsies)
Specification Kidney biopsies
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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Submitted Sep 19 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Mar 30 2012

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 20 µg
Specification HEK293T
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Mar 30 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver biopsy)
Specification Liver biopsy
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Saira Khalid

Verified customer

Submitted Apr 07 2010

Application Western blot
Sample Human Cell lysate - other (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 18 2008

Application Western blot
Sample Human Cell lysate - nuclear (SupT1, Jurkat, Hela cells)
Loading amount 15 µg
Specification SupT1, Jurkat, Hela cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Nov 08 2006

The antibody has good chances of working in other types of immunohistochemistry other than those already tested as it has the ability to recognise the native form of the protein. If the customer means IF as in ICC/IF I would recommend to try fixing t...

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Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 21 2005

All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain...

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All the information we have for this product for use in IHC is on the datasheet, including recommended dilutions and advice for antigen retrieval. I also suggest that you click through to the review submitted by a researcher who successfully used this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"