Overview

  • Product nameAnti-MCM2 antibody [EPR4120]See all MCM2 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4120] to MCM2
  • Tested applicationsWB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • WB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa cell lysates IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    This product is available conjugated to DyLight® 488 see ab139902.

Properties

Applications

Our Abpromise guarantee covers the use of ab108935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 102 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • Sequence similaritiesBelongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    Anti-MCM2 antibody [EPR4120] images

    • All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : Molt-4 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 102 kDa
    • ab108395, at a 1/250 dilution, staining MCM2 in paraffin-embedded Human squamous cell cervical carcinoma tissue
    • ab108395, at a 1/250 dilution, staining MCM2 in paraffin-embedded Human tonsil tissue
    • ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-MCM2 antibody [EPR4120] (ab108935)

    ab108935 has not yet been referenced specifically in any publications.

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