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A synthetic di-phosphorylated peptide, corresponding to a sequence from human MCM2 surrounding Serines 40 and 41. (NP_004517.2)
Our Abpromise guarantee covers the use of ab70371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 100 kDa).|
|Flow Cyt||Use 0.1µg for 106 cells.
0.1 μg per 1 X 106 cells in a 150 µl volume.
Ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab70371 at 0.1µg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x106 cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.
ab70371 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"