Overview

  • Product name
  • Description
    Rabbit polyclonal to MCM3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 42-272 of Human MCM3 (NP_002379).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; Cal27 xenograft; 293T, A431, H1299, Molt-4 and Raji cell lines

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cervical cancer proto oncogene 5 antibody
    • DNA polymerase alpha holoenzyme associated P1 antibody
    • DNA polymerase alpha holoenzyme associated protein P1 antibody
    • DNA polymerase alpha holoenzyme-associated protein P1 antibody
    • DNA replication factor MCM3 antibody
    • DNA replication licensing factor mcm3 antibody
    • HCC 5 antibody
    • HCC5 antibody
    • hRlf beta subunit antibody
    • Human cervical cancer proto oncogene 5 antibody
    • MCM 3 antibody
    • mcm3 antibody
    • MCM3 minichromosome maintenance deficient 3 antibody
    • MCM3_HUMAN antibody
    • MGC1157 antibody
    • Minichromosome maintenance complex component 3 antibody
    • Minichromosome maintenance deficient 3 antibody
    • Minichromosome maintenance protein 3 antibody
    • P1 h antibody
    • P1 MCM3 antibody
    • P1 Protein antibody
    • P1-MCM3 antibody
    • P1.h antibody
    • p102 antibody
    • P102 protein antibody
    • Replication licensing factor beta subunit antibody
    • RLF beta subunit antibody
    • RLF subunit beta antibody
    • RLFB antibody
    see all

Images

  • Anti-MCM3 antibody (ab97282) at 1/10000 dilution + HepG2 whole lysate at 30 µg

    Predicted band size : 91 kDa
  • Immunofluorescence analysis of MCM3 in paraformaldehyde-fixed HeLa cells, using ab97282 at 1/500 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of MCM3 in paraffin-embedded Cal27 xenograft, using ab97282 at 1/500 dilution.

References

ab97282 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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