Synthetic peptide (Human) - 38 amino acids which represent a portion of human MCM 3 encoded in part by exons 10 and 11.
Our Abpromise guarantee covers the use of ab4460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 90 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 19073720|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|