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- MCM3 antibody (ab4460)
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Western blot - MCM3 antibody (ab4460)
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Product Name
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MCM3 antibody - ChIP Grade
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See all MCM3 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCM3 - ChIP Grade
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Immunogen
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Synthetic peptide (Human) - 38 amino acids which represent a portion of human MCM 3 encoded in part by exons 10 and 11.
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Reacts with
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Hu, Chk
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Tested applications
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ChIP, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ChIP: Use at an assay dependent dilution (MCM3 binding to EBV in Raji cells, PubMed: 19073720).
IP: Use at a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000. Detects a band of approximately ~105 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
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MCM3 antibody - ChIP Grade (ab4460) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCM3 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCM3 antibody - ChIP Grade images, references, products related to ab4460 and other tools.
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MCM3 antibody - ChIP Grade images:
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 - MCM3 antibody (ab4460)
Predicted band size : 90 kDa
HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour.
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Western blot - MCM3 antibody (ab4460)
Predicted band size : 90 kDa
Western blot analysis of ab4460 on HeLa OHIO extract.
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References for MCM3 antibody - ChIP Grade (ab4460)
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This product has been used in: (two most recent references)
Bair AM et al. Ca2+ Entry via TRPC Channels Is Necessary for Thrombin-induced NF-{kappa}B Activation in Endothelial Cells through AMP-activated Protein Kinase and Protein Kinase C{delta}. J Biol Chem 284:563-74 (2009). WB; Human. PubMed: 18990707
Zhou J et al. Epstein-Barr virus episome stability is coupled to a delay in replication timing. J Virol 83:2154-62 (2009). ChIP; Human. PubMed: 19073720
See all 4 publication references for this product.
If you publish research using ab4460 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM3
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MCM3 antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding MCM3 antibody - ChIP Grade |
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MCM3 antibody - ChIP Grade - related products:
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MCM3 antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about MCM3 antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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