Anti-MCM4 antibody (ab3728)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-MCM4 antibody
See all MCM4 primary antibodies -
DescriptionGoat polyclonal to MCM4
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Chicken, Human
Predicted to work with: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.
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Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
Target
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FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
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Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
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Alternative names
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-MCM4 antibody (ab3728)Sample: Hela cell RIPA extract (60
µ g)Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60
µg) Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
References
This product has been referenced in:
- Tenca P et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007). Read more (PubMed: 17062569) »
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human, Chicken . Read more (PubMed: 17635936) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"