Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.
Our Abpromise guarantee covers the use of ab3728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"