• Product nameAnti-MCM4 antibody
    See all MCM4 primary antibodies
  • Description
    Rabbit polyclonal to MCM4
  • Specificityab74237 detects endogenous levels of total MCM4 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).

  • Positive control
    • HepG2 cell extracts. Human testis tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab74237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.


  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 19G antibody
    • AI325074 antibody
    • AU045576 antibody
    • CDC 21 antibody
    • CDC 54 antibody
    • CDC21 antibody
    • CDC21 homolog antibody
    • CDC21, S. pombe, homolog of antibody
    • CDC54 antibody
    • Cell division cycle 21, S. pombe, homolog of antibody
    • DNA replication licensing factor MCM 4 antibody
    • DNA replication licensing factor MCM4 antibody
    • hCdc 21 antibody
    • hCdc21 antibody
    • Homolog of S. pombe cell devision cycle 21 antibody
    • KIAA4003 antibody
    • mcdc21 antibody
    • MCM 4 antibody
    • MCM 4 minichromosome maintenance deficient 4 antibody
    • mcm4 antibody
    • MCM4 minichromosome maintenance deficient 4 antibody
    • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
    • MCM4_HUMAN antibody
    • Mcmd4 antibody
    • MGC33310 antibody
    • Minichromosome maintenance 4 antibody
    • Minichromosome maintenance complex component 4 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
    • Minichromosome maintenance deficient 4 antibody
    • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
    • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
    • mKIAA4003 antibody
    • NKCD antibody
    • NKGCD antibody
    • P1 CDC21 antibody
    • P1-CDC21 antibody
    • P1CDC21 antibody
    see all

Anti-MCM4 antibody images

  • ab74237 at 1/50 dilution staining MCM4 in human testis by Immunohistochemistry using Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-MCM4 antibody (ab74237) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HepG2 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
    Additional bands at : 120 kDa,84 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MCM4 antibody (ab74237)

ab74237 has not yet been referenced specifically in any publications.

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