Recombinant
RabMAb

Anti-MCM4 antibody [EPR5245] (ab124836)

Overview

  • Product name
    Anti-MCM4 antibody [EPR5245]
    See all MCM4 primary antibodies
  • Description
    Rabbit monoclonal [EPR5245] to MCM4
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • Positive control
    • HeLa, Molt-4, and A431 cell lysates and Molt-4 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.80 x 10 -11 M
    Learn more about KD
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5245
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • Sequence similarities
      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 19G antibody
      • AI325074 antibody
      • AU045576 antibody
      • CDC 21 antibody
      • CDC 54 antibody
      • CDC21 antibody
      • CDC21 homolog antibody
      • CDC21, S. pombe, homolog of antibody
      • CDC54 antibody
      • Cell division cycle 21, S. pombe, homolog of antibody
      • DNA replication licensing factor MCM 4 antibody
      • DNA replication licensing factor MCM4 antibody
      • hCdc 21 antibody
      • hCdc21 antibody
      • Homolog of S. pombe cell devision cycle 21 antibody
      • KIAA4003 antibody
      • mcdc21 antibody
      • MCM 4 antibody
      • MCM 4 minichromosome maintenance deficient 4 antibody
      • mcm4 antibody
      • MCM4 minichromosome maintenance deficient 4 antibody
      • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
      • MCM4_HUMAN antibody
      • Mcmd4 antibody
      • MGC33310 antibody
      • Minichromosome maintenance 4 antibody
      • Minichromosome maintenance complex component 4 antibody
      • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
      • Minichromosome maintenance deficient 4 antibody
      • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
      • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
      • mKIAA4003 antibody
      • NKCD antibody
      • NKGCD antibody
      • P1 CDC21 antibody
      • P1-CDC21 antibody
      • P1CDC21 antibody
      see all

    Images

    • All lanes : Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Molt-4 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 97 kDa
    • Flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green(negative).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124836 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124836.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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