Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)
Key features and details
- Mouse monoclonal [CRCT5.1 (A2.7A3)] to MCM5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]
See all MCM5 primary antibodies -
Description
Mouse monoclonal [CRCT5.1 (A2.7A3)] to MCM5 -
Host species
Mouse -
Specificity
Recognises human Mcm-5 -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MCM5.
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Positive control
- Cervical Carcinoma Tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CRCT5.1 (A2.7A3) -
Myeloma
Sp2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4174 Human
- Omim: 602696 Human
- SwissProt: P33992 Human
- Unigene: 517582 Human
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Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab6154 has been referenced in 6 publications.
- Lin JX et al. CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1a/VEGFA signaling. Cancer Cell Int 19:282 (2019). PubMed: 31728130
- Mishra S et al. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell 174:803-817.e16 (2018). PubMed: 30057114
- Yin Y & Rothenberg E Probing the Spatial Organization of Molecular Complexes Using Triple-Pair-Correlation. Sci Rep 6:30819 (2016). PubMed: 27545293
- Munday DC et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). PubMed: 25355874
- Rakotomalala L et al. Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy. J Biol Chem 283:28729-40 (2008). ICC/IF ; Mouse . PubMed: 18693245
- Williams GH et al. Improved cervical smear assessment using antibodies against proteins that regulate DNA replication. Proc Natl Acad Sci U S A 95:14932-7 (1998). PubMed: 9843993