Overview

  • Product name
    Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]
    See all MCM5 primary antibodies
  • Description
    Mouse monoclonal [CRCT5.1 (A2.7A3)] to MCM5
  • Specificity
    Recognises human Mcm-5
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Bacterially expressed human Mcm5

  • Positive control
    • Cervical Carcinoma Tissue
  • General notes
    Clone Number: CRCT5.1 (A2.7A3)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    CRCT5.1 (A2.7A3)
  • Myeloma
    Sp2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18693245

Target

  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

Images

  • ab6154 staining MCM5 in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Munday DC  et al. Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis. J Virol 89:917-30 (2015). Read more (PubMed: 25355874) »
  • Rakotomalala L  et al. Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy. J Biol Chem 283:28729-40 (2008). ICC/IF ; Mouse . Read more (PubMed: 18693245) »

See all 3 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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