Anti-MCM5 antibody [EP2682Y] (ab76023)
Key features and details
- Rabbit monoclonal [EP2682Y] to MCM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-MCM5 antibody [EP2682Y]
See all MCM5 primary antibodies -
Description
Rabbit monoclonal [EP2682Y] to MCM5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF7 and human spleen lysates IHC-P: human squamous cell cervical carcinoma tissue, normal tonsil tissue, Glioma tissue, Colonic adenocarcinoma tissue, Endometrial carcinoma tissue and Ovarian carcinoma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2682Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 82 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 82 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4174 Human
- Omim: 602696 Human
- SwissProt: P33992 Human
- Unigene: 517582 Human
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Alternative names
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
Images
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All lanes : Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa -
ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing positive staining in Glioma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing positive staining in Endometrial carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab76023 showing positive staining in Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76023 has been referenced in 1 publication.
- Zhang R et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). Human . PubMed: 27863397