Overview

  • Product name
    Anti-MCM5 antibody [EP2682Y]
    See all MCM5 primary antibodies
  • Description
    Rabbit monoclonal [EP2682Y] to MCM5
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human MCM5

  • Positive control
    • HeLa, MCF7 and human spleen lysates; human squamous cell cervical carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP2682Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 82 kDa.
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

Images

  • All lanes : Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.
  • ab76023 showing positive staining in Normal tonsil tissue.

  • ab76023 showing negative staining in Normal brain tissue.

  • ab76023 showing positive staining in Glioma tissue.

  • ab76023 showing positive staining in Colonic adenocarcinoma tissue.

  • ab76023 showing positive staining in Endometrial carcinoma tissue.

  • ab76023 showing positive staining in Ovarian carcinoma tissue.

References

This product has been referenced in:
  • Zhang R  et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). Human . Read more (PubMed: 27863397) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76023.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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