Overview

  • Product nameAnti-MCM5 antibody [EP2682Y]
    See all MCM5 primary antibodies
  • Description
    Rabbit monoclonal [EP2682Y] to MCM5
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human MCM5

  • Positive control
    • HeLa, MCF7 and human spleen lysates; human squamous cell cervical carcinoma tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP2682Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 82 kDa.
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

Anti-MCM5 antibody [EP2682Y] images

  • All lanes : Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.
  • ab76023 showing positive staining in Normal tonsil tissue.

  • ab76023 showing negative staining in Normal brain tissue.

  • ab76023 showing positive staining in Glioma tissue.

  • ab76023 showing positive staining in Colonic adenocarcinoma tissue.

  • ab76023 showing positive staining in Endometrial carcinoma tissue.

  • ab76023 showing positive staining in Ovarian carcinoma tissue.

References for Anti-MCM5 antibody [EP2682Y] (ab76023)

ab76023 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76023.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"