Overview

  • Product nameAnti-MCM5 antibody [EP2683Y]
    See all MCM5 primary antibodies
  • Description
    Rabbit monoclonal [EP2683Y] to MCM5
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MCM5 aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P33992

  • Positive control
    • HeLa and MCF7 cell lysates; human squamous cell cervical carcinoma tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.
    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-MCM5 antibody (Alexa Fluor® 405) [EP2683Y] (ab210141)
    Anti-MCM5 antibody (Alexa Fluor® 647) [EP2683Y] (ab210425)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP2683Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 46 antibody
    • CDC46 antibody
    • CDC46 homolog antibody
    • Cell division cycle 46 antibody
    • DNA replication licensing factor antibody
    • DNA replication licensing factor MCM 5 antibody
    • DNA replication licensing factor MCM5 antibody
    • MCM 5 antibody
    • MCM5 antibody
    • MCM5_HUMAN antibody
    • MGC5315 antibody
    • Minichromosome maintenance complex component 5 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 5 antibody
    • Minichromosome maintenance deficient 5 antibody
    • Minichromosome maintenance deficient protein 5 antibody
    • P1 CDC46 antibody
    • P1-CDC46 antibody
    see all

Anti-MCM5 antibody [EP2683Y] images

  • ab75975 showing positive staining in Normal tonsil tissue.

  • Immunofluorescence staining of MCF7 cells with purified ab75975 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
  • ab75975 at 1/250 dilution staining MCM5 in human squamous cell cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • ab75975 showing negative staining in Normal prostate tissue.

  • ab75975 showing positive staining in Prostatic carcinoma tissue.

  • ab75975 showing positive staining in Glioma tissue.

  • ab75975 showing positive staining in Breast carcinoma tissue.

  • ab75975 showing positive staining in Endometrial carcinoma tissue.

  • ab75975 showing positive staining in Colonic adenocarcinoma tissue.

References for Anti-MCM5 antibody [EP2683Y] (ab75975)

This product has been referenced in:
  • Drissi R  et al. Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage. Mol Cell Proteomics 14:2002-13 (2015). Human . Read more (PubMed: 25963833) »
  • Hsu I  et al. Suppression of ERß signaling via ERß knockout or antagonist protects against bladder cancer development. Carcinogenesis N/A:N/A (2013). IHC ; Mouse . Read more (PubMed: 24148819) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab75975.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"