Overview

  • Product name
  • Description
    Rabbit polyclonal to MCM6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    KYLQLAEELIRPERNT

    conjugated to KLH, corresponding to amino acids 45-60 of Mouse MCM6.

  • Positive control
    • Human Tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Images

  • ab115350, at 1/50 dilution, staining MCM6 in formalin-fixed, paraffin-embedded Human Tonsil tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115350 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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