Overview

  • Product nameAnti-MCM6 antibody
    See all MCM6 primary antibodies
  • Description
    Goat polyclonal to MCM6
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21980527

Target

  • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

Anti-MCM6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM6 with ab3730 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 115 kDa

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

     

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

  • Immunofluorescence analysis of rat kidney tissue collected at 0 hours (shame) and 24 hours after ischemic injury, and embryonic rat kidney tissue from embryonic day 17 (E17d).

    MCM6 (red) was stained using ab3730 at 1/1000 dilution. Nuclei were stained with DAPI (blue).

References for Anti-MCM6 antibody (ab3730)

This product has been referenced in:
  • Kim TM  et al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr ; Rat . Read more (PubMed: 21980527) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab3730.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"