Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.
Our Abpromise guarantee covers the use of ab4458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sample: HeLa cell RIPA extract (50
Antibody: ab4458 at 0.2