Overview

  • Product name
  • Description
    Rabbit polyclonal to MCM6
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.

Properties

Applications

Our Abpromise guarantee covers the use of ab4458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
    • Sequence similarities
      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Binds to chromatin during G1 and detach from it during S phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA replication licensing factor MCM6 antibody
      • MCG40308 antibody
      • Mcm6 antibody
      • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
      • MCM6 minichromosome maintenance deficient 6 antibody
      • MCM6_HUMAN antibody
      • Minichromosome maintenance complex component 6 antibody
      • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
      • Minichromosome maintenance deficient 6 homolog antibody
      • Minichromosome maintenance protein 6 antibody
      • Mis5 antibody
      • MIS5 homolog antibody
      • p105MCM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection: DAB.


    • Predicted band size : 91 kDa

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
    • IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Quimbaya M  et al. Deregulation of the Replisome Factor MCMBP Prompts Oncogenesis in Colorectal Carcinomas through Chromosomal Instability. Neoplasia 16:694-709 (2014). Human . Read more (PubMed: 25246271) »
    • Gronnier C  et al. The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells. Biochim Biophys Acta 1843:2432-7 (2014). Read more (PubMed: 25003315) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human cerebral organoid)
    Specification
    Human cerebral organoid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Radhika Menon

    Verified customer

    Submitted Dec 13 2017

    Thanks for your enquiry. Unfortunately, we are not aware of any publications that feature the use of either of these antibodies. We also have not received any feedback from customers in the form of reviews who have used these antibodies. For both...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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