Anti-MCM7 antibody [47DC141 or DCS-141] (Biotin) (ab79802)

Overview

  • Product nameAnti-MCM7 antibody [47DC141 or DCS-141] (Biotin)
    See all MCM7 primary antibodies
  • Description
    Mouse monoclonal [47DC141 or DCS-141] to MCM7 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis
  • Immunogen

    Recombinant full length Human MCM7 protein

  • Positive control
    • MAD109, or PC12 cells. Tonsil or breast cancer tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number47DC141 or DCS-141
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab79802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 0.5 - 1.0 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Native and denatured.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 81 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
    • Sequence similaritiesBelongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDABP0042 antibody
      • CDC 47 antibody
      • CDC47 antibody
      • CDC47 homolog antibody
      • Cdc47, S. cerevisiae, homolog of antibody
      • DNA replication licensing factor MCM7 antibody
      • Homolog of S. cerevisiae Cdc47 antibody
      • MCM 2 antibody
      • MCM 7 antibody
      • MCM2 antibody
      • MCM2, formerly antibody
      • Mcm7 antibody
      • MCM7 minichromosome maintenance deficient 7 antibody
      • MCM7_HUMAN antibody
      • Minichromosome Maintainence 7 antibody
      • Minichromosome maintainence, S. cerevisiae, homolog of antibody
      • Minichromosome maintenance complex component 7 antibody
      • Minichromosome maintenance deficient 7 antibody
      • Minichromosome maintenance protein 7 antibody
      • P1.1 MCM3 antibody
      • P1.1-MCM3 antibody
      • P1CDC47 antibody
      • P85MCM antibody
      • PNAS 146 antibody
      • PNAS146 antibody
      see all

    References for Anti-MCM7 antibody [47DC141 or DCS-141] (Biotin) (ab79802)

    This product has been referenced in:
    • Albuquerque AV  et al. Spermatogonial behavior in rats during radiation-induced arrest and recovery after hormone suppression. Reproduction 146:363-76 (2013). Read more (PubMed: 23884860) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"