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- Specific References
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Overview
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Product nameAnti-MCM7 antibody
See all MCM7 primary antibodies -
DescriptionRabbit polyclonal to MCM7
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Rat, Human
Does not react with: Mouse -
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human MCM7.
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Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab51062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | ||
| WB |
Application notes
IP: 1/20.
WB: 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
Is unsuitable for ICC/IF or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
Is unsuitable for ICC/IF or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
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Sequence similaritiesBelongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4176 Human
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: P33993 Human
- Unigene: 438720 Human
- Unigene: 113 Rat
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Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Images
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Western blot - Anti-MCM7 antibody (ab51062)Anti-MCM7 antibody (ab51062) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDa
References
ab51062 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"