Overview

  • Product name
  • Description
    Rabbit polyclonal to MCM7
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human MCM7.

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    IP: 1/20.
    WB: 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

    Is unsuitable for ICC/IF or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
    • Sequence similarities
      Belongs to the MCM family.
      Contains 1 MCM domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDABP0042 antibody
      • CDC 47 antibody
      • CDC47 antibody
      • CDC47 homolog antibody
      • Cdc47, S. cerevisiae, homolog of antibody
      • DNA replication licensing factor MCM7 antibody
      • Homolog of S. cerevisiae Cdc47 antibody
      • MCM 2 antibody
      • MCM 7 antibody
      • MCM2 antibody
      • MCM2, formerly antibody
      • Mcm7 antibody
      • MCM7 minichromosome maintenance deficient 7 antibody
      • MCM7_HUMAN antibody
      • Minichromosome Maintainence 7 antibody
      • Minichromosome maintainence, S. cerevisiae, homolog of antibody
      • Minichromosome maintenance complex component 7 antibody
      • Minichromosome maintenance deficient 7 antibody
      • Minichromosome maintenance protein 7 antibody
      • P1.1 MCM3 antibody
      • P1.1-MCM3 antibody
      • P1CDC47 antibody
      • P85MCM antibody
      • PNAS 146 antibody
      • PNAS146 antibody
      • PPP1R104 antibody
      • Protein phosphatase 1, regulatory subunit 104 antibody
      see all

    Images

    • Anti-MCM7 antibody (ab51062) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 81 kDa
      Observed band size : 81 kDa

    References

    ab51062 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab51062.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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