• Product name
  • Description
    Rabbit polyclonal to MCM8
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: LRDAPEKTLA CMGLAIHQVL TKDLERHAAE LQAQEGLSND GETMVNVPHI, corresponding to N terminal amino acids 145-194 of Human MCM8

  • Positive control
    • Jurkat cell lysate



Our Abpromise guarantee covers the use of ab66761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 78, 82 kDa (predicted molecular weight: 93 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.


  • Function
    Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.
  • Tissue specificity
    Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
  • Sequence similarities
    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localization
    Nucleus. Localizes to nuclear foci and colocalizes with RAD51.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf154 antibody
    • dJ967N21.5 antibody
    • DNA helicase MCM8 antibody
    • DNA replication licensing factor MCM8 antibody
    • MCM8 antibody
    • MCM8_HUMAN antibody
    • MGC119522 antibody
    • MGC119523 antibody
    • MGC12866 antibody
    • MGC4816 antibody
    • Minichromosome maintenance 8 antibody
    • Minichromosome maintenance complex component 8 antibody
    • REC antibody
    see all

Anti-MCM8 antibody images

  • Anti-MCM8 antibody (ab66761) at 0.125 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MCM8 antibody (ab66761)

ab66761 has not yet been referenced specifically in any publications.

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