Overview

  • Product nameAnti-MCM8 antibody
    See all MCM8 primary antibodies
  • Description
    Rabbit polyclonal to MCM8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 144 - 193 (ELRDAPEKTL ACMGLAIHQV LTKDLERHAA ELQAQEGLSN DGETMVNVPH) of Human MCM8 (NP_115874)

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.
  • Tissue specificityHighest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.
  • Sequence similaritiesBelongs to the MCM family.
    Contains 1 MCM domain.
  • Cellular localizationNucleus. Localizes to nuclear foci and colocalizes with RAD51.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf154 antibody
    • dJ967N21.5 antibody
    • DNA helicase MCM8 antibody
    • DNA replication licensing factor MCM8 antibody
    • MCM8 antibody
    • MCM8_HUMAN antibody
    • MGC119522 antibody
    • MGC119523 antibody
    • MGC12866 antibody
    • MGC4816 antibody
    • Minichromosome maintenance 8 antibody
    • Minichromosome maintenance complex component 8 antibody
    • REC antibody
    see all

Anti-MCM8 antibody images

  • Anti-MCM8 antibody (ab86169) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa

References for Anti-MCM8 antibody (ab86169)

ab86169 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86169.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"