Our Abpromise guarantee covers the use of ab21396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 5 - 10 µg/ml. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.
Flow Cyt Use 0.5µg for 106 cells. in 100µl volume for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP1 producing cells within mixed cell populations.

ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
IHC-FoFr Use at an assay dependent concentration. Samples need to be saponin-treated.


  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody [2H5] images

  • Ab21396 staining human MCP-1 by ELISA. Image shows a standard curve obtained with increasing concentrations of human MCP-1.

References for Anti-MCP1 antibody [2H5] (ab21396)

This product has been referenced in:
  • Zhang Y  et al. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol 168:3088-98 (2002). Read more (PubMed: 11884483) »
  • Luo Y  et al. Chemokine amplification in mesangial cells. J Immunol 163:3985-92 (1999). Read more (PubMed: 10491001) »

See all 3 Publications for this product

Product Wall

Below is a link to a paper that uses clone 2H5 (same clone as ab21396) for acetone fixed, frozen tissue staining (Figure 8).


Thank you for contacting us.

As shown on the following SwissProtlink, http://www.uniprot.org/uniprot/P14844, the calculated molecular weight of the Rat MCP1 is 16.4 kDa, not 11 kDa as for Human. The differences in the molecular weight observed...

Read More

Vielen Dank für Ihre Anrufe.
Wie besprochen können wir den MCP1 Antikörper für Western blot und Durchflußzytometrie garantieren (www.abcam.com/Abpromise). In der Immuncytochemie/Immunfluoreszenz (ICC/IF) wurde er noch nicht getestet, aber es beste...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (aotic endothelial cells)
Specification aotic endothelial cells
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type Indirect

Abcam user community

Verified customer

Submitted Jan 31 2012

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. I am glad to learn that the customer expects expression of the protein in these tissues, because following good scientific practise a posit...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this Anti-MCP1 antibody. We have received no complaints about this product therefore I a...

Read More

Thank you for your enquiry. I have checked with our laboratory and it seems that this product has not been tested in paraffin embedded sections. The application that we have stated on the datasheet refers to frozen sections, and tissue/cells grown ...

Read More

Thank you for your enquiry. The following paper has a brief description of a procedure for staining frozen sections of rat retina with MCP-1 antibody. It is not the same antibody as our hamster antibody but the procedure is standard. The important ...

Read More

Thank you for your reply. A MCP-1 positive control in tissue is a bit tricky as production usually tracks with disease states (for example trauma or autoimmune disease). One of the best positive controls for MCP-1 in tissue is the central nervous ...

Read More