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Our Abpromise guarantee covers the use of ab25124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information).
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|Neutralising||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 25 kDa.|
IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.