• Product nameAnti-MCP1 antibody
    See all MCP1 primary antibodies
  • Description
    Rabbit polyclonal to MCP1
  • Specificityab25124 reacts with both rat MCP1 and its mouse homolog JE.
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Rat MCP1 aa 1-73 (N terminal).


  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Rat Kidney.



Our Abpromise guarantee covers the use of ab25124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Neutralising Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 25 kDa.


  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody images

  • IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution

    Lane 1 : 40ug whole rat spinal cord protein lysate
    Lane 2 : 40ug whole mouse spinal cord protein lysate
    Lane 3 : Naive Rat
    Lane 4 : Sham 1
    Lane 5 : Sham 2
    Lane 6 : Injured 1
    Lane 7 : Injured 2
    Lane 8 : Injured 3

    Goat anti-Rabbit HRP
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
    This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.

    See Abreview

References for Anti-MCP1 antibody (ab25124)

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Aoki K  et al. Role of Indoxyl Sulfate as a Predisposing Factor for Atrial Fibrillation in Renal Dysfunction. J Am Heart Assoc 4:e002023 (2015). WB, IHC . Read more (PubMed: 26452986) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Oct 14 2015

Application Western blot
Sample Human Recombinant protein (Recombinant protein)
Loading amount 0.2 µg
Specification Recombinant protein
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 17 2010

Thank you for your enquiry. We recommend that the azide be dialyzed out of the antibody preparation before any in vitro or in vivo studies.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Primary Mixed Cortical Cultures)
Specification Primary Mixed Cortical Cultures
Permeabilization No

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Apr 12 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Spinal Cord)
Specification Spinal Cord
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ms. Nancy Nutile-McMenemy

Verified customer

Submitted Dec 07 2005

Thank you for your enquiry. This antiserum was tested by western blotting using RAW cells treated with LPS for 15 hours. 10ug of lysate and 10ug of supernatant was loaded onto the gel. A prominent band of 25 kDa was detected. I hope this informat...

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