Anti-MCP1 antibody (ab7202)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, Neutralising
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-MCP1 antibody
See all MCP1 primary antibodies -
Description
Rabbit polyclonal to MCP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, Neutralisingmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Rat MCP1.
Database link: P14844 -
Positive control
- IHC-P: LPS-treated rat lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
1/300.
|
|
IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 21088249
|
WB | (1) |
1/2000. Predicted molecular weight: 18 kDa.
|
Neutralising |
Use at an assay dependent concentration.
|
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/300. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 21088249 |
WB
1/2000. Predicted molecular weight: 18 kDa. |
Neutralising
Use at an assay dependent concentration. |
Target
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 290320 Mouse
- Unigene: 4772 Rat
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Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
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All lanes : Anti-MCP1 antibody (ab7202)
Lane 1 : LPS-stimulated RAW cell lysate
Lane 2 : Rat MCP1-transfected RAW cell lysate
Predicted band size: 18 kDaWestern blot analysis of RAW cell lysates labelling MCP1 with ab7202.
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Immunohistochemical analysis of paraffin-embedded LPS-treated rat lung tissue stained for MCP1 using ab7202 at 1/300 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (99)
ab7202 has been referenced in 99 publications.
- Hu Y et al. Potential Molecular Mechanism of Yishen Capsule in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Molecular Docking. Diabetes Metab Syndr Obes 15:943-962 (2022). PubMed: 35378831
- Zhou M et al. Post-Stroke Administration of L-4F Promotes Neurovascular and White Matter Remodeling in Type-2 Diabetic Stroke Mice. Front Neurol 13:863934 (2022). PubMed: 35572941
- Lee AR et al. Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis. Immune Netw 22:e14 (2022). PubMed: 35573148
- Luo Q et al. Long Noncoding RNA MEG3-205/Let-7a/MyD88 Axis Promotes Renal Inflammation and Fibrosis in Diabetic Nephropathy. Kidney Dis (Basel) 8:231-245 (2022). PubMed: 35702702
- Mao M et al. Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis. Evid Based Complement Alternat Med 2022:8547095 (2022). PubMed: 35754699