Overview

  • Product name
    Anti-MCP1 antibody
    See all MCP1 primary antibodies
  • Description
    Rabbit polyclonal to MCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Rat).

  • Positive control
    • RAW 264.7 cells (mouse macrophage/monocyte adherent cell line) stimulated with LPS for 15 hours

Properties

Applications

Our Abpromise guarantee covers the use of ab7202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 21088249
ICC/IF Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 18 kDa.

Target

  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    Images

    References

    This product has been referenced in:
    • Meng CY  et al. Cytochrome P450 26A1 modulates natural killer cells in mouse early pregnancy. J Cell Mol Med 21:697-710 (2017). IHC-P ; Mouse . Read more (PubMed: 27860312) »
    • Takahashi Y  et al. Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma. Oncol Lett 14:264-270 (2017). Read more (PubMed: 28693163) »

    See all 48 Publications for this product

    Publishing research using ab7202? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    16 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-MCP1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    30 µg
    Specification
    liver
    Treatment
    2mg/kg LPS 1h
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 16 2012

    I am a Ph.D. student doing research on the effects of obesity on the placental development. Unfortunately the antibodies of interest have not been tested in the uterus/placenta. I am writing to see if your company would willing to give us a small sampl...

    Read More

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

    Read More

    Thanks for your kindly reply, the information of this product as follow:

    Lot: gr47175-3

    Po: 1051535

    And after I contacted with this customer, she would like to have a credit note, therefore, could you please help her to...

    Read More

    Thank you for your note.



    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab7202. For your information, the internal reference note for...

    Read More

    Dear abcam technical support team:

    This customer has purchase ab7202 (Anti-MCP1 antibody) and has conducted the WB several times with mouse sample.

    The results show no band; moreover, this customer also has used RAW 264.7 cells an...

    Read More

    Thank you for your enquiry regarding ab7202 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

    The protocol looks fine to me. It may wel...

    Read More

    No signal in ELISA with ab20641. No signal in WB with ab7202.

    Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on th...

    Read More

    Nuclear staining in mouse spleen and lymph node.

    Thank you for your call today and for your questions about ab7202. As we discussed, we recommend using a microwave antigen retrieval method with this antibody. However, if the antibody is still staining the wrong localization, please let me know a...

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-MCP1 antibody

     Poor
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (injred spinal cord tissue)
    Specification
    injred spinal cord tissue
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    Username

    Ms. soo ryung jeong

    Verified customer

    Submitted Oct 01 2009

    BATCH NUMBER 7052 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Western blot: no bands detectable Immunohistochemistry: no reaction SAMPLE WB: mouse aorta protein extract IHC: mouse frozen cross sections of the ao...

    Read More

    I'm sorry to hear the customer is having problems with his new vial of ab7202. Thank you for taking the time to fill in the questionnaire, I can see clearly that the protocol looks very good and the antibody should perform if the customer has previousl...

    Read More

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 57806 DESCRIPTION OF THE PROBLEM low signal and high background using a biotinylated secundary antibody non-signal using a FITC-conjugated secondary antibody SAMPLE Aortic frozen sect...

    Read More

    I'm sorry to hear you are having problem with ab7102. We have not tested it in mouse aorta or have any reviews yet from customers using it in aorta. So please do let us know when your staining works and we will reward you with an Amazon gift voucher (a...

    Read More

    Customer called about what of fixative to use for IHC-Frozen sections.

    Thank you for your phone call. There are no special conditions that need to be followed to use ab7202 in IHC-frozen sections and so I suggest fixing your sections with acetone as per our general IHC protocol for frozen sections (the protocol can be fou...

    Read More

    1-10 of 16 Abreviews or Q&A

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