Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCAF/MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 0.5 µg/ml. To detect hMCAF/MCP-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCAF/MCP-1.
Neutralising Use a concentration of 3 - 5 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of hMCAF/MCP-1 (10.0 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20369226

Target

  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody images


  • Developed using the ECL technique

    Performed under reducing conditions.

    HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/20000 dilution
    Developed using the ECL technique

    Observed band size : 12-14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Dr Laura Toma by Abreview.

    See Abreview

  • ab9669 at 2.5 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P.  The anitbody was incubated for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. 
  • Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.

Protocols

References for Anti-MCP1 antibody (ab9669)

This product has been referenced in:
  • Sindhu S  et al. Obesity Is a Positive Modulator of IL-6R and IL-6 Expression in the Subcutaneous Adipose Tissue: Significance for Metabolic Inflammation. PLoS One 10:e0133494 (2015). IHC-P ; Human . Read more (PubMed: 26200663) »
  • Yang S  et al. Defective mitophagy driven by dysregulation of rheb and KIF5B contributes to mitochondrial reactive oxygen species (ROS)-induced nod-like receptor 3 (NLRP3) dependent proinflammatory response and aggravates lipotoxicity. Redox Biol 3:63-71 (2014). WB ; Rat . Read more (PubMed: 25462067) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - other (Endothelial cells (EA.hy926) - conditioned media)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Loading amount 500000 cells
Treatment 100 µg/ml glycated LDL (AGE-LDL) for 24 hours
Specification Endothelial cells (EA.hy926) - conditioned media
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Anca Sima

Verified customer

Submitted Jun 18 2015

Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either se...

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Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please kee...

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Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab...

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Thank you for your reply.

Please keep me updated on any progress. I hope that we hear something from them soon.

Please let me know if you need anything else from me at this time, and I'll let you know if I hear from someone at BioCo...

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Thank you again for your patience.

At this point, I have not heard from Bio-Connect regarding these issues and I've looked through our system and don't see that they've corresponded with anyone else at Abcam. Have you received any update from...

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Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out wi...

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Thank you for your reply and for confirming that information.

I'm processing the refunds and replacements now. I think I've found your order for ab22048, ab9100, and ab8021, but I am having some trouble finding the order for ab9669. Were thes...

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Thank you for getting back to me with your selections.

For CX3CR1, we do have a couple of other anti-human antibodies that have been tested in IHC on FFPE sections, ab8020 and ab51668-

http://www.abcam.com/CX3CR1-antibody-ab8020.htm...

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Thank you for getting back to me, and I am happy to help resolve this issue. I look forward to hearing from you, and please let me know if you would like any assistance selecting replacements, or if you have any other questions.

Have a great ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"