Anti-MCP1 antibody (ab9858)

Overview

• Product nameAnti-MCP1 antibodySee all MCP1 primary antibodies ...
• Description
  Mouse monoclonal to MCP1
• Tested applicationsELISA, Sandwich ELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant hMCAF/MCP-1 (human Macrophage/Monocyte chemotactic protein-1)

Properties

• FormLyophilised:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPhosphate buffered saline
• Concentration information loading...
• PurityProtein A purified
• Purification notesPurified from ascitic fluid
• Clonality Monoclonal
• Myelomaunknown
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Chemokines

  • Immunology
  • Innate Immunity
  • Chemokines
  • Alpha Chemokines (CXC)

Applications

Target

• FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
• Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
• Post-translational
  modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
• Cellular localizationSecreted.

Target information above from: UniProt accession P13500 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6347 Human
  • Omim: 158105 Human
  • SwissProt: P13500 Human
  • Unigene: 303649 Human

• Alternative names
  C-C motif chemokine 2 antibodyCCL 2 antibodyCCL2 antibody

  CCL2_HUMAN antibodyChemokine (C C motif) ligand 2 antibodyChemokine C C motif ligand 2 antibodyChemokine CC Motif Ligand 2 antibodyGDCF 2 antibodyGDCF 2 HC11 antibodyGDCF-2 antibodyGDCF2 antibodyHC11 antibodyHSMCR30 antibodyHSMCR30 antibodyJE antibodyMCAF antibodyMCP 1 antibodyMCP-1 antibodyMCP1 antibodyMGC9434 antibodyMonocyte chemoattractant protein 1 antibodyMonocyte chemotactic and activating factor antibodyMonocyte chemotactic and activating factor antibodyMonocyte chemotactic protein 1 antibodyMonocyte secretory protein JE antibodyMonocyte secretory protein JE antibodySCYA2 antibodySmall inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibodySmall inducible cytokine A2 antibodySmall inducible cytokine subfamily A (Cys Cys), member 2 antibodySmall inducible cytokine subfamily A Cys Cys member 2 antibodySmall-inducible cytokine A2 antibodySMC CF antibodySMC-CF antibodySMCCF antibody

  see all

Anti-MCP1 antibody images

• 

  Sandwich ELISA - MCP1 antibody (ab9858)
  Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCP1 antibody (ab9858)

  ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Protocols

Protocol Booklet