Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.2 - 0.4 µg/ml.

Target

  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • Chemokine C C motif ligand 2 antibody
    • Chemokine CC Motif Ligand 2 antibody
    • GDCF 2 antibody
    • GDCF 2 HC11 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small inducible cytokine subfamily A Cys Cys member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody images

  • Standard curve for MCP1 (Analyte: ab73866); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5µg/ml.
  • ab9858 staining MCP1 in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 10 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Protocols

References for Anti-MCP1 antibody (ab9858)

This product has been referenced in:
  • Ji WT  et al. Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma. PLoS One 9:e88952 (2014). IHC-P ; Human . Read more (PubMed: 24586454) »
  • Tripathy D  et al. Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia. Front Aging Neurosci 5:19 (2013). IHC-P ; Mouse . Read more (PubMed: 23675346) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% Gel)
Sample Human Tissue lysate - whole (adipose tissue)
Specification adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Ashley Davies

Verified customer

Submitted Feb 03 2014

Thank you for your recent enquiry.

I have confirmed with our goods out department that this item was reconstituted before sending out to you.

Therefore, you will not need to do any further reconstitution, and I can suggest to ensu...

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Thank you for contacting Abcam.

All of the IHC information that we have for ab9858 is available on the website, below is a copy of that protocol information:

"ab9858 staining MCP1 in human breast invasive ductal carcinoma tissu...

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Thank you for your inquiry about the clone number for ab9858. I'm really sorry about the delay in giving you this information, I had to contact the source. We currently hold 078Y023 for ab9858 therefore, the clone number is PEPY023_hMCP1. I hope thi...

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There is no known clone number for this antibody

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"