Anti-MCP1 antibody [2.2-4H5-1A11] (ab9858)
Key features and details
- Mouse monoclonal [2.2-4H5-1A11] to MCP1
- Suitable for: WB, IHC-P, ELISA, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
-
Product name
Anti-MCP1 antibody [2.2-4H5-1A11]
See all MCP1 primary antibodies -
Description
Mouse monoclonal [2.2-4H5-1A11] to MCP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ELISA, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human MCP1. Recombinant hMCAF/MCP-1 (human Macrophage/Monocyte chemotactic protein-1)
Database link: P13500 -
Positive control
- WB: Recombinant human MCP1 protein (ab9670).
-
General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab73680
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2.2-4H5-1A11 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
Sandwich ELISA |
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669).
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection. |
Notes |
---|
WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP1 (ab9669). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9669 as Detection. |
Target
-
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
-
Alternative names
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
Images
-
In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Datasheets and documents
-
Datasheet download
References (4)
ab9858 has been referenced in 4 publications.
- Migacz E et al. Elevation of CD40/CD40L Inflammatory Pathway Molecules in Carotid Plaques from Moderate-and-Severe Obstructive Sleep Apnea Patients. Diagnostics (Basel) 11:N/A (2021). PubMed: 34067481
- Luo S et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). PubMed: 29725470
- Ji WT et al. Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma. PLoS One 9:e88952 (2014). IHC-P ; Human . PubMed: 24586454
- Tripathy D et al. Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia. Front Aging Neurosci 5:19 (2013). IHC-P ; Mouse . PubMed: 23675346